Helicase Ded1 fine-tunes the reprogramming of gene expression conferred by inhibition of TORC1 by rapamycin.

The role of Ded1 helicase is pivotal in reducing ribosomal biogenesis and inducing autophagy under TORC1 inhibition. Incorporating mutations in Ded1 rescues these effects, leading to rapamycin resistance. Overall design: This study includes a total of 36 samples: 18 for ribosome profiling and 18 for RNA-Seq, derived from triplicates of WT Ded1, ded1-?N, and ded1-?C untreated and rapamycin-treated cells. All strains were grown in YPD at 30ºC until the OD600 reached approximately 0.4. They were then grown further for 2 hr with or without rapamycin (200ng).