muscle and white adipose tissue transcriptomes in obese WT mice after 12 weeks of prednisone regimens

Exogenous glucocorticoids regulate the muscl-fat axis communication, but little attention is paid to their frequency of intake. Here we investigated the transcriptional effects of prednisone in muscle and adipose tissue when dosed as intermittent once-weekly 1mg/kg i.p. versus once-daily 1mg/kg in WT obese mice. Prior to treatment, mice were fed a high-fat diet for 12 weeks. After 12 weeks of treatment, we performed RNA-seq analysis in muscle (quadriceps) and white adipose tissue (ventral fat pad) to quantitate gene expression at isoform level. 3 muscle samples vehicle; 3 muscle samples weekly prednisone; 3 muscle samples daily prednisone; 3 adipose samples vehicle; 3 adipose samples weekly prednisone; 2 adipose samples daily prednisone. Each sample was ~1ug RNA isolated through Trizol and further purifed through RNeasy columns.