Transcriptome sequencing of Bipolaris oryzae to study regulation of its gene

The transcriptome sequencing of B. oryzae was performed to study its gene regulation in two contrasting rice host. For sequencing of control fungus, fungal mycelial block was grown in potato dextrose medium in three replicates and RNA with RIN value >7 was isolated and processed for pair end (150 nucleotide read length) sequencing. For infection related RNA sequencing, samples with three replicates at four time points were collected by infecting two rice cultivars with contrasting responses identified earlier i.e Gitesh (moderately resistant) and Shahsarang (susceptible) was sequenced (Marwein et al., 2022) . The sequence can be accessed from following BioProject: PRJNA650013