Bradyrhizobium sp. INPA03-11B Genome sequencing. Bradyrhizobium sp. INPA03-11B

The strain INPA03-11B, isolated in the 1980s from nodules of Centrosema sp. collected in the Manaus area, Amazonas, Brazil, was approved by the Brazilian Ministry of Agriculture as a cowpea inoculant in 2004. Since then, several studies have been conducted regarding its phenotypic, genetic, and symbiotic characteristics. Several phenotypic features demonstrate its high adaptability to stressful soil conditions, such as tolerance to acidity, high temperatures, and antibiotics, and in symbiotic characteristics, mainly its high efficiency with cowpea and soybean, proven in the field. The sequencing of the 16S rRNA ribosomal gene and housekeeping genes, as well as BOX-PCR profiles, showed its potential as a new species, which was confirmed by a similarity percentage of 94.6 in Average Nucleotide Identity with the closest phylogenetically related species - Bradyrhizobium tropiciagri. Therefore, we suggest the name Bradyrhizobium amazonense for INPA3-11B, representing the type strain of this new species.