Neutralizing antibodies induced by recombinant EMC viruses in mice.
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a Eight-week-old male BALB/c mice (n = 5) were intraperitoneally immunized with 5×104 PFU recombinant virus and T1100I in 0.2 ml 2% DMEM. Mice (n = 3) inoculated with 0.2 ml 2% DMEM in the same route were used as negative control.b Neutralization antibody titers were defined as the reciprocal of highest serum dilution that neutralized 100 TCID50 of JXwn06 and were expressed as the geometric mean value of 5 mice.c Neutralization antibody titers were defined as the reciprocal of highest serum dilution that neutralized 200 TCID50 of CSFV C-strain and were expressed as the geometric mean value of 5 mice.d Serum samples were collected at day 0 before immunization.e Mice were boosted with identical dose (5×104 PFU) of either viruses or medium at 14 dpi.Neutralizing antibodies induced by recombinant EMC viruses in mice.
