Molecular characterization of aged human eccrine gland cells using single-cell transcriptomic analysis

To uncover the natural cell heterogeneity state of healthy eccrine sweat gland (ESG) at the gene level, we obtained normal human skin tissue from three subjects without clinical evidence or a history of inflammatory or systemic skin disease and isolated the ESG performed single-cell (sc) RNA sequencing assay. Overall design: The ESGs were stained with neutral red and then isolated from human skin tissue under a stereomicroscope using forceps.Then single cells of isolated ESGs were prepared and analyzed using scRNAseq.