Frequency of immune cells in PBMC and TBLN of Nano-KAg vaccinated pigs.

Pigs were unvaccinated or vaccinated with either K-Ag or Nano-KAg once intranasally and challenged with PRRSV strain MN184 and euthanized at DPC 15. Different immune cell subsets present in PBMC and TBLN MNC were enumerated by flow cytometry. a CD172+ cells were gated to enumerate CD11c and SLAII expression and the percent of DCs rich fraction (CD172+CD11c+SLAII+) is shown. b CD3− cells were gated to enumerate CD4 and CD8α expression and the percent NK cell rich fraction (CD3−CD4− CD8α+) is shown. c CD25+ cells were gated to enumerate CD4 and Foxp3 expression and the percent of CD4+CD25+Foxp3+ cell is shown. Each number is an average percent of immune cells from three pigs +/− SEM. Asterisk represents the statistical significant difference (p