The numerical values underlying Figs 1F and 3D and 4E and 5G–5I and S7C and S7D and S8B and S8C.

Sheets show the values underlying the data displayed in all figures and include an explanation of all data. Sheet Fig 1F shows the raw data from in vitro substrate fluorescence assays for WT LACTB underlying Fig 1F. Sheet Fig 3D details the raw data from in vitro substrate fluorescence assays for LACTB mutants underlying Fig 3D. Sheet Fig 4E describes the raw data from in vitro substrate fluorescence assays for LACTB mutants underlying Fig 4E. Sheet Fig 5G shows the raw data from in vitro substrate fluorescence assays in the presence of liposomes underlying Fig 5G. Sheet Fig 5H describes the raw data from LACTB membrane-binding assays based on membrane composition underlying Fig 5H. Sheet Fig 5I reports the raw data from LACTB membrane-binding assays for LACTB mutants underlying Fig 5I. Sheet S7C Fig represents the raw data from LACTB membrane-binding assays based on membrane composition underlying S7C Fig. Sheet S7D Fig shows the raw data from LACTB membrane binding assays for WT LACTB based on binding classification underlying S7D Fig. Sheet S8B Fig details the raw data from in vitro substrate fluorescence assays for LACTB mutants underlying S8B Fig. Sheet S8C Fig describes the raw data from LACTB membrane binding assays for LACTB mutants underlying S8C Fig.
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