TABLE 1 in Murichromolaenicola thailandensis sp. nov. (Phaeosphaeriaceae, Dothideomycetes) from Chromolaena odorata (Asteraceae) in northern Thailand

TABLE 1. Gene regions, primers and PCR amplification conditions used in this study.
GenePrimers ForwardReversePCR ConditionsReferencesLarge subunit (LSU)LR0RLR595 °C: 3 min, (94 °C: 30 s, 56 °C: 50 s, 72 °C: 1 min) × 40 cycles 72 °C: 7 min.Vilgalys & Hester (1990)Small subunit (SSU)NS1NS495 °C for 3 min, (94 °C: 30 s, 55 °C: 50 s, 72 °C:1 min) × 40 cycles 72 °C: 7 min.White et al. (1990)Internal transcribed spacer (ITS)ITS5ITS495 °C for 3 min, (94 °C: 30 s, 55 °C: 50 s, 72 °C:1 min) × 40 cycles 72 °C: 7 min.White et al. (1990)Elongation factor-1 alpha (tef1- α)EF-1 983FEF1-2218R95 °C: 3 min, (94 °C: 30 s, 55 °C: 50 s, 72 °C: 1 min) × 40 cycles 72 °C: 7 min.Rehner (2001)RNA polymerase II subunit (rpb2)fRPB2-5 FfRPB2-7cR95 °C: 5 min, (95 °C:1 min, 52 °C: 2 min, 72 °C: 90 s) × 40 cycles 72 °C: 10 minLiu et al. (1999)