Expression profiling following Nrf2D29H or Keap1R554Q expression in mouse embryonic fibroblasts

We generated mouse embryonic fibroblasts from genetically engineered mouse models harboring conditional alleles of Nfe2l2(Nrf2)D29H and Keap1R554Q and induced expresison of these mutations using adenoviral Cre. Gene expression was profiled. Overall design: MEFs were isolated at embryonic day 13.5-14.5. Homozygous Nrf2D29H or Keap1R554Q MEFs were infected with empty adenovirus (control) or adenoviral cre to induce expression of the mutations. Keap1 was functionally wild-type in adenoviral mock conditions, and homozygous mutant after Ad-Cre infection. Nrf2 was functionally knock-out prior to infection and in adenoviral-mock cells, and homozygous D29H after infection with adenoviral Cre. MEFs were collected 4 days after infection for RNA.