DNA methylation of two heat-stress genes in redband trout from constrasting thermal environments. Oncorhynchus mykiss gairdneri

Epigenetic variation is a potential pathway for rapid response to environmental change and may influence local adaptation at the population level through population by environment interactions. Here, we focus on the most well-understood mechanism of epigenetic variation, DNA-methylation. Previous lab studies have shown consistent ecotypic variation in DNA-methylation, but few have attempted to quantify epigenetic variation in natural populations. We developed a study to compare levels of DNA-methylation in two heat-stress related genes in redband trout (Oncorhynchus mykiss gairdneri) from contrasting environments. We sampled trout populations from cold montane and warm desert streams at repeated intervals, collecting tissue samples that were used for epigenetic analysis and levels of DNA-methylation (percent methylation) were quantified using targeted bisulfite sequencing.