Location of NFIA-ETO2 binding site on genome in MEL cells

The NFIA-ETO2 fusion is the product of a t(1;16)(p31;q24) chromosomal translocation so far exclusively found in pediatric patients with pure erythroid leukemia (PEL). To address its role for the pathogenesis of the pure erythroid leukemia, we retrovirally expressed the PEL-associated NFIA-ETO2 fusion in MEL cells. To better identify direct target gene loci, we sequenced NFIA-ETO2-associated immunoprecipitated chromatin and histone modifications. Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for HA NFIA-ETO2 tag, IGG and histone modifications H3K27ac, H3K4me1, H3K27me3 and H3K9me1 in MEL cells expressing or not NFIA-ETO2.