Transcriptomic analysis of antigen experienced Mariyln (TCR-transgenic) CD4 T cells proliferation during an ongoing immune response in vivo. Transcriptomic analysis of antigen experienced Mariyln (TCR-transgenic) CD4 T cells proliferation during an ongoing immune response in vivo

The study explore the intrinisic inhibitors of antigen experienced Marilyn CD4 T cells (TCR-Tg) expansion after footpad vaccinations in vivo and in the presence of a first cohort of expanded Marilyn cells to mimick an ongoing immune response Overall design: C57BL6 mice are intravenously injected with either naive or antigen experienced Marilyn cells and restimulated via footpad vaccinations (DC+peptide) in the presence of the first cohort of Marilyn cells, mimicking an ongoing CD4 immune response. CD4 T cells are sorted from draining lymph node, 7 days after transfer.