Transcriptional profiling of Bone Marrow Derived Dendritic Cells (BMDC) stimulated with zymosan or bacterial spent medium. Transcriptional profiling of Bone Marrow Derived Dendritic Cells (BMDC) stimulated with zymosan or bacterial spent medium

We reported transcriptional characterization of BMDCs stimulated by filter-sterilized spent medium of Parabacteroides goldsteinii ASF519 (ASF519 SSM) alone or in comination with zymosan. We identified that ASF519 SSM-induced differentially expressed genes have a wide association with the regulation of immune functions, cell communication, apoptosis. We observed that ASF519 SSM induced transcriptomic changes towards immune tolerance, such as a dramatic induction of tolerogenic Il10, lipocalin-2(Lcn2) and indoleamine 2, 3-dioxygenases (Ido1/2), and a reduction of the co-stimulatory molecule Cd40 and inflammatory cytokines, including Il12b, Il23a, and Il1f9 (or Il-36 gamma). Overall design: Bulk RNA sequencing of BMDCs post stimulation with either ASF519 SSM or uninoculated bacterial broth alone, or in combination with zymosan.