Flow cytometry analysis of freshly isolated cells from the ocular surface.

Data is presented as mean ±standard deviation of 5–6 different experiments per group/time point. Lymphocytes were gated based on characteristic light-scatter properties (“gated cells”), subsequently gated based on forward scatter height vs. forward scatter area and propridium iodide live/dead exclusion (“live gated cells”). Data presented represents percentage of positive (+) or negative (-) cells after background subtraction.NS = non-stressed, DS5 = desiccating stress for 5 days, DS10 = desiccating stress for 10 days.