Comparative liver proteomic analysis of proteins expression changes involved in the regulation of phagocytosis in a mouse model of acute-on-chronic liver failure

The definitive molecular mechanisms underlying the complex pathological processes of ACLF has not yet been clarified. In the current study, we established a mouse model of ACLF by intraperitoneal administration of carbon tetrachloride (CCl4) coupled with lipopolysaccharide (LPS) and D-galactosamine (D-Gal) and explored the underlying molecular pathogenesis of ACLF development. 4D label-free mass spectrometry-based quantitative proteomics was used to identify liver proteins differentially expressed in liver samples from CCl4 coupled with LPS/D-Gal-induced ACLF mouse models.