H1NMR data of Chemical structure of compounds

Both compounds were tested for purity using RP-HPLC with acetonitrile–water (1:5 v/v). Compound 1 showed a signal at a retention time of 15.87 min with some minor signals. Compound 2 showed a signal at a retention time of 21.06 min with some minor signals. Compounds were identified by their H1NMR data comparing with those recorded in the literature. The structure corresponding to compound 1 is presented in Figure 5 and agrees with the chemical structure of a catechin. Optical rotation of compound 1 was [α]20 = -53,4; which allows us to conclude that compound 1 is (-)-epicatechin. The structure corresponding to compound 2, from extract of Hypnea musciformis, is presented in Figure 6 and agrees with the chemical structure of a dihydrochalcone known as phloretin.