Analysis of transgenic Anopheles gambiae lines with perturbed expression of the primary sex determiner gene Yob. Analysis of transgenic Anopheles gambiae lines with perturbed expression of the primary sex determiner gene Yob

The study aims at understanding the genome-wide transcriptomic response in the African malaria mosquito Anopheles gambiae to a perturbance in expression of the primary sex determination gene Yob. The study encompassed third instar larvae and approximately 24 h-old pupae of (i) a transgenic A. gambiae line (called 23) females with an ectopic Yob expression, phenotypically manifested by a female masculinization and death during late development, (ii) male progeny from a cross between males stably expressing single guide RNA targeting Yob and females expressing Cas9 in the germline, and as a control for comparisons (iii) the wild-type G3 strain mosquitoes of the same age. The RNA samples were sequenced using Illumina NovaSeq6000 platform.