Activity of rationally constructed variants
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aThe parental subtilisin donating the sequence to each of the 6 regions is superscripted.bThe activity of the cell culture medium against substrate Suc-Phe-Ala-Ala-Phe-pNA. ND is not determined.cThe activity of the cell culture medium against substrate Suc-Ala-Ala-Pro-Phe-pNA. Cells marks with ‘–’ indicates no observable activity. ND is not determined.dThe ratio of the initial rates for the two substrates for each variant. Those marked with ‘–’ could not be calculated. ND is not determined.
创建时间：
2015-12-02
