Ratios RI, FP & EC

Quantification of mixed ratios of different gut-bacterial pairs: Roseburia intestinalis + Faecalibacterium prausnitzii (RI-FP), Roseburia intestinalis + Escherichia coli with mCherry expressed (RI-EC), Faecalibacterium prausnitzii + Escherichia coli with mCherry expressed (FP-EC) Notes: Initial monocultures of the three strains were measured and all monocultures were diluted in PBS to the same concentration. Subsequently, the monocultures were mixed in different ratios (5%-95%, as described in the sample names). In addition, monoculture ratios were also made in PBS (5%-95%) to approximate cell counts in the mixtures.