Nuclear envelope-associated lipid droplets are enriched in cholesteryl esters and increase during inflammatory signaling

Volume electron microscopy datasets (SBF-SEM and FIB-SEM) of human epithelial carcinoma A431 cells and human primary macrophages.

The datasets described in
Szkalisity Á, Vanharanta L, Saito H, Vörös C, Li S, Isomäki A, Tomberg T, Strachan C, Belevich I, Jokitalo E, Ikonen E.
Nuclear envelope-associated lipid droplets are enriched in cholesteryl esters and increase during inflammatory signaling
EMBO J. 2025 May;44(10):2774-2802. doi: 10.1038/s44318-025-00423-2. Epub 2025 Apr 7.
PMID: 40195500
https://doi.org/10.1038/s44318-025-00423-2

SBF-SEM: Serial Block-Face Scanning Electron Microscopy
FIB-SEM: Focused Ion Beam Scanning Electron Microscopy

FIB-SEM datasets:
240729_HumanMP_TNFa_1U
    A differentiated primary human macrophage treated with tumor necrosis factor alpha (TNFa) for 1 h.
240801_HumanMP_Cont
    A primary human macrophage grown in control differentiation medium.

SBF-SEM datasets:
231106_A431_CLEM_CM1_3J, Run: 04_R01
    An A431 cell grown in complete medium.
240306_OA-Chol_7T, Run:03_R02_7T
    A differentiated primary human macrophage loaded with 200 μM cholesterol and 200 μM oleic acid for 2 hours.
240307_OA-Chol_AV, Run: 03_R01_AV-3
    A differentiated primary human macrophage loaded with 200 μM cholesterol and 200 μM oleic acid for 2 hours.
240307_OA-Chol_AV, Run: 04_R02_AV-1
    A differentiated primary human macrophage loaded with 200 μM cholesterol and 200 μM oleic acid for 2 hours.
240424_Microphages_no_load, Run: 03_R02_1
    A primary human macrophage grown in control differentiation medium.