Sheep gastric organoids and Hco-miR-5352

Infection with helminth parasites is a significant health and economic problem in humans and livestock. Helminths can modulate host immune response, mainly via release of excretory-secretory (ES) products. While effects of individual ES proteins have been characterised, the impact of secreted microRNAs (miRNAs) on host gene expression and immune outcome remains unclear. Nematodes infecting the gastrointestinal (GI) tract are closely associated with host epithelial cells, including tuft cells, which initiate a type 2 response, and mucous secreting cells, which are responsible for the weep and sweep response to expel GI nematodes. These RNA-seq results detail the effect of a pan GI-nematode secreted miRNA on host epithelial cells using gastric (sheep) organoids. Ovine abomasum organoids were exposed to four different conditions for 48 h before RNA extraction and sequencing i) Abomasum organoids (CONTROL) ii) Stimulated with IL-13 (IL13) iii) Stimulated with IL-13 and transfected with miRNA-mimic-control (CONTROL MIMIC) iv) Stimulated with IL-13 and transfected with miR-5352-mimic (MIMIC). Four biological replicates each were used (n=4).