Characterization of lncRNA and mRNA Profiles in Rats With Diabetic Macroangiopathy

Diabetic macroangiopathy is one of the most common serious complications of diabetes. Previous studies indicate that lncRNAs involved in the process of diabetes and another vascular disease. However, their detailed mechanism of the lncRNAs involved in diabetic macroangiopathy has not been well characterized. In the present study, we generated rat models of diabetic macroangiopathy induced by High fat. A total of 15 GK rats was constructed as a test group, along with 15 Wistar rats set as a control group, and thoracic aorta tissue from each group was collected. Whole genomic RNA sequencing was performed on thoracic aorta tissue;3223 novel lncRNAs and 20367 annotated lncRNAs were identified in thoracic aorta samples, and 864 lncRNAs were expressed differently in the test and control groups. Gene ontology term enrichment showed the apparent enrichment of inflammatory response and cell apoptosis, which consistent with the results of H&E Staining, TUNEL Assay, and ELISA; Extensive literature reveals inflammatory response and cell apoptosis play an important role in the process of diabetic macroangiopathy.The results of the present study indicated that lncRNAs, especiallyNrep.bSep08,Col5a1,aSep0,soygee.aSep08-unspliced,NONRATT013247.2,votar.aSep08-unspliced,etc,both participate in and mediate the process of inflammatory response,cell apoptosis.What’s more, Our research provides further insights into the understanding of the basic molecular mechanisms underlying diabetic macroangiopathy. Our research showed that characterization of lncRNA and mRNA Profiles in Rats With Diabetic Macroangiopathy by High-throughput sequencing technology which was used to obtain the differential mRNA/lncRNA expression profiles of the thoracic aorta of each group of rats, further screen the differential mRNA/differential lncRNA target gene collection, and use GO enrichment and KEGG enrichment for biological analysis. Interpret the characterization of lncRNA and mRNA Profiles in Rats With Diabetic Macroangiopathy from the molecular level.