Transcriptional profiling of basilar artery and basilar terminus intima and media in rabbits after bilateral carotid ligation. Transcriptional profiling of basilar artery and basilar terminus intima and media in rabbits after bilateral carotid ligation

New Zealand white rabbits underwent bilateral carotid ligation to increase flow in the basilar artery (BA) or sham surgery. 24 hours post-surgery, the BA and basilar terminus (BT) were embedded for sectioning. The intima and media were separately laser-microdissected from the sections, and whole transcriptome RNA-seq was performed on each tissue in order to more thoroughly characterize potential molecular signals between endothelium and smooth muscle during flow-induced arterial remodeling. Overall design: Female New Zealand white rabbits underwent bilateral common carotid artery ligation surgery (n=2) or a sham surgery (n=2). After 24 hours, vascular sections of the basilar artery (n=4) and the basilar terminus (n=4) were embedded for sectioning. The intima (n=8 samples) and media (n=8 samples) were separately laser-microdissected from the sections. Whole transcriptome RNA-seq was performed on each tissue sample (n=16).