Predicted and observed TRF sizes using restriction enzymes Alu-I and Hinf-I.

Genotype identities refer to those given in Figure 1 and accession numbers in the European Molecular Biology Laboratory Nucleotide Sequence Database. Predicted terminal restriction fragment (TRF) sizes are the lengths of fragments (in base pairs; bp) generated by in silico digestion of sequences of each genotype. Observed TRFs are those found from the empirical analysis of artificial community templates. TRFs which were produced by empirical TRFLP analysis of individual genotypes and have therefore been combined in further analyses are given in the same row. Alu-I TRFs refer to HEX labelled fragments and Hinf-I to FAM labelled fragments.Predicted and observed TRF sizes using restriction enzymes Alu-I and Hinf-I.