Data from: ATP13A2 is involved in intracellular polyamine transport in lung epithelial cells

Polyamines are present in all living cells and are implicated in various
crucial cellular processes such as proliferation, apoptosis, and
autophagy, while excess amounts of polyamines can be toxic to cells.
ATP13A2 was recently identified as a mammalian polyamine transporter in
neuronal cells, and in this study, we attempted to characterize the
function of ATP13A2 in cultured human lung epithelial cells. The data
demonstrate that ATP13A2 is endogenously expressed in the mouse lung, and
in cultured lung epithelial cells, the expression level of ATP13A2
drastically changes over culture time and peaks at logarithmic phase
during the proliferation curve. ATPase activity in ATP13A2-enriched
microsomes from lung cells showed polyamine-dependence, as previously
reported in other cell types and species. Overexpression of ATP13A2 caused
a moderate increase in total cellular polyamine content, while genetic
ablation of ATP13A2 resulted in a decrease in cellular polyamine levels.
These findings provide novel information regarding the cellular function
of ATP13A2 in the lung and contribute to the understanding of the cellular
polyamine transport system.