Z. Xu, H. Ogawa, P. Vagnarelli, J.H. Bergmann, D.F. Hudson, S. Ruchard, T. Fukagawa, W.C. Earnshaw, K. Samejima (2011) CIL:12172, Gallus gallus gallus, memory B cell. CIL. Dataset
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Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Metaphase cell localization of exogenous TrAP-INCENP (red) plus endogenous CENP-A (green) and DNA (blue) in INCENP OFF cells expressing mutant TrAP-INCENP F802A. See Fig 6C panel 9 in Xu et al. 2009 J Cell Biol 187, 637-653.
DT40鸡B淋巴细胞系细胞在有丝分裂时的细胞图像,采用Deltavision系统,以100x/1.4NA计划阿波镜头进行宽场免疫荧光成像,并利用约束迭代算法(Applied Precision)进行去卷积处理。图中展示的是中央z切片。展示了在表达突变型TrAP-INCENP F802A的INCENP OFF细胞中,外源TrAP-INCENP(红色)与内源CENP-A(绿色)以及DNA(蓝色)的中间期细胞定位。详见Xu等人在2009年《细胞生物学杂志》第187卷第637-653页的第6C部分第9图。
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CIL



