meg-3 meg-4 mutants are defective in exogenous RNAi signal amplification

Worms were fed a dsRNA trigger against the pos-1 gene and subjected to sRNAseq to see if they could amplify the exogenous RNAi trigger. meg-3 meg-4 were not able to amplify the signal as robustly as wild-type. Worms were starvation synchronized and plated on bacteria expressing dsRNA corresponding to the pos-1 gene. Samples were collected at the young mother stage, and subject to sRNAseq.