Mitochondrial iron transport via MFRN1 is required for erythroid cell cycle progression

The goal of the scRNAseq study was to identify developmental defects and differential gene expression in MFRN1 mutant d3 zebrafish embryos Overall design: 10 Tg(lcr:GFP) and Tg(lcr:GFP); MFRN1 mutant 3 dpf zebrafish embryos of the AB strain were homogenized and subjected to scRNAseq anlysis. GFP is under the control of the globin promoter and its expression is restricted to the erythroid lineage. The goal was to identify lineage defects (erythroid and other) that resulted from MFRN1 deficiency, as well as identify differnetially expressed genes.