Superoxide release by colostrum phagocytes (mean ± SD, N = 8 per treatment).

Phagocytes were incubated with enterophatogenic Escherichia coli (EPEC) in the presence of melatonin (MLT), N-acetyl-serotonin (NAS), luzindole or chloromelatonin (CMLT) and pre-treated or not with 8-(Diethylamino) octyl-3,4,5-trimethoxybenzoate hydrochloride (TMB-8). In controls assays, phagocytes were pre-incubated with phosphate buffer solution (PBS).*indicates difference from the control treatment (ANOVA, P+indicates intergroup differences within each treatment (ANOVA, P#indicates differences between TMB-8 use within each treatment and group.