Measurement of A-to-I editing in various adult mouse brain areas in SNORD115-expressing and SNORD115-deficient specimens

The SNORD115 snoRNA has been proposed to regulate A-to-I editing of the 5-HT2C pre-mRNA. We prepared a mouse strain where the SNORD115 locus is deleted. Because that snoRNA locus is submitted to genomic imprinting (only the paternal allele is expressed), we compared WT specimens (which express SNORD115) to paternally-inherited deletions (which do not express SNORD115). For each dissected brain area in each replicate, the edited segment of 5-HT2C mRNA was amplified by RT-PCR and deep-sequenced.