Data analysis for IDR-1002 cytotoxicity assays.

Figure E1. Cytotoxicity assessment of IDR-1002 in HepG2 and BEC cells using the MTT assay. HepG2 (A) and BEC cells (B) were treated for 8 h with 1, 10, 25, 50, and 100 µM IDR-1002 or with 10 µM tert-butyl hydroperoxide (TBHP) as a positive control for cytotoxicity. Cell viability was assessed using the MTT assay and expressed as a percentage relative to the untreated control (CTRL). No significant differences in viability were observed between IDR-1002-treated groups and untreated control, indicating that IDR-1002 is not cytotoxic at the tested concentrations. In contrast, TBHP significantly reduced cell viability. Comparisons not indicated by asterisks were not statistically significant (ns). Data represents the mean ± standard deviation (SD) from three independent experiments (n = 3). Statistical analysis was performed using two-way ANOVA followed by Tukey’s post hoc test (***p