RecombiCraft library construction: A novel method for DNA library cloning and expansion using non-enzymatic single-step DNA recombination and liquid culture
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a HA-tag coding sequence is in italics with a TAA stop codon in bold at the 3’ end of Ha-tag, restriction enzyme sites are underlined. b The location of each primer cited in the table was accorded to
Herein we propose a fully in vitro iterative method based on Golden Gate Aseembly to faithfully construct a long synthetic DNA molecule starting from commercially available double-stranded DNA. The sy