Reliance of neuronal gene expression on cohesin scales with chromatin loop length (5C). Reliance of neuronal gene expression on cohesin scales with chromatin loop length (5C)

5C-seq from Rad21lox/lox;NexCre/+ and Rad21+/+;NexCre/+ dissociated cortical neurons at Day In Vitro (DIV10) without stimulation or after treatment with TTX/D-AP5 and 1h of KCl depolarization. And, 5C of and doxycycline treated Rad21-TEV primary neurons transduced with NLS-TEV Overall design: 5C-seq from Rad21lox/lox;NexCre/+ and Rad21+/+;NexCre/+ dissociated cortical neurons at Day In Vitro (DIV10) without stimulation or after treatment with TTX/D-AP5 and 1h of KCl depolarization. And, Total of 8 samples analysed; 2 replicates of Rad21-tev primary neurons 24h following doxycyline treatment (6 hours, 100ng/ml), 2 replicates of Rad21-tev primary neurons 24h following vehicle treatment. 2 replicates of Rad21-tev primary neurons 7 days following doxycycline treatment (6 hours, 100ng/ml), 2 replicates of Rad21-tev primary neurons 7 days following vehicle treatment.