RNA-seq of in vitro differentiated naïve and Th9 cells

To investigate changes in gene expression over time, we differentiated Th9 cells in vitro from naïve T cells from human and murine sources, and followed them over an extended time course, during which they gained and then lost capacity for IL-9 production We then performed gene expression profiling analysis using data obtained from RNA-seq in cells from 2 different human donors and from 2 mice at 4 time points. Comparative gene expression profiling analysis of RNA-seq data for d0 (naïve), d3, d4. and d8 Th9 cells (mouse) or d0 (naïve), d5, d8, and d15 Th9 cells (human)