Effect of HBV on pDC maturation.
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To investigate possible pDC activation by HepG2.215-derived HBV in comparison with other pDC stimuli, PBMC were cultured in the presence or absence of HBV (100 geq/cell), CpG, HSV-1, Lox or Influenza (see ‘ctr’ lane). To investigate the immune regulatory effect of HBV on pDC maturation induced by other known pDC activating ligands, PBMC were cultured with or without CpG, HSV-1, Lox or Influenza in the presence (‘HBV’ lane) or absence (‘ctr’ lane) of HBV (100 geq/cell). After 24h, cells were harvested and the expression of CD40, CD80, CD86 and HLA-DR on pDC was determined by flow cytometry. Data are presented as mean±SEM fluorescent intensity of 3 independent experiments with different donors. Similar data were observed for purified pDC (not shown).*p
创建时间：
2015-12-02
