Effect of hypoxia and atpenin A5 on the human monocyte transcriptome. Hypoxia, atpenin A5 and human monocyte transcriptome

Monocyte-enriched peripheral blood mononuclear cells of apparently healthy human blood donors were cultured under normoxia or hypoxia (1% O2) for 24 hours at a high density (>20 million/ml). Normoxic cultures were also treated with 1 uM atpenin A5 for 48 hours. Afterwards, CD14+ monocytes were isolated using magnetic beads and their transcriptomes examined by sequencing rRNA-depleted total RNA (Illumina TruSeq Stranded Total RNA library preparation kit; HiSeq 2000 sequencer; paired 101 b reads; 6 libraries per flow lane).