Reproducibility of detecting differences in dystrophin expression in DMD muscle biopsies by immunofluorescence using MANDYS106 antibody and Definiens intensity measurements (at high laser settings).

The intra-assay precision (CV%) was calculated for the mean dystrophin intensity of multiple sections of a biopsy/sample analyzed in the same experiment. Inter-assay precision between experiments performed on different days was assessed in two ways by ranking of the samples based on the dystrophin intensity in each experiment and by calculating the CV% from the mean dystrophin intensity in each experiment (different experiments 3, 4, 5, 6, and 7 were performed over the course of 1.5 years with the day of experiment shown in the table).CV, coefficient of variation; DMD, Duchenne muscular dystrophy.Reproducibility of detecting differences in dystrophin expression in DMD muscle biopsies by immunofluorescence using MANDYS106 antibody and Definiens intensity measurements (at high laser settings).