Assembled mRNAs from potato genotypes contrasted for their hexanal isomerase activity
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From PacBio and Illumina mRNA-seq coming from leaf total RNA isolated from three <em>Solanum tuberosum</em> genotypes (Desiree, Phureja153 and S869), mRNA isoforms were assembled. <strong>Phureja153:</strong> low (3Z):(2E) hexenal isomerase activity <strong>Desiree:</strong> mid (3Z):(2E) hexenal isomerase activity <strong>S869</strong>: high (3Z):(2E) hexenal isomerase activity <strong>== Step1: PacBio subreads to FASTA and GTF annotation == </strong> For each genotype, a FASTA file and a GTF annotation file are available. File is called "pacbio_assembled_isoforms.tar.gz". Pipeline used: https://github.com/SilkeAllmannLab/pacbio_snakemake/releases/tag/v0.1.0 Raw PacBio data submitted to NCBI EBI ENA under accession PRJXXX. === Step 2: SQANTI QC ==== SQANTI3 was run on these three PacBio assembled sets of transcript to correct them. The corrected transcripts and related SQANTI3 info are available in the "sqanti_corrected_transcripts.tar.gz" file.



