Expression data of S1M cells sorted high and low after ST2 antibody treatment

ST2 functions as a receptor for the cytokine IL-33. It has been implicated in carcinogenesis. In this study, we sought to mechanistically determine how ST2 and IL-33 function to support cancer stem cell (CSC) activity and drive gastric cancer (GC) pathogenesis. We used microarrays to detail the global program of gene expression among S1M ST2 high and low cells and to identify distinct classes of genes upregulated in this process. ST2 high and low cells (2 samples) were successfully isolated using a Flow Cytometry Sorter and selected at a continuous stage of early development for RNA extraction and hybridization on Affymetrix microarrays.