Inflammatory Disease Progression Shapes Nanoparticle Biomolecular Corona-Mediated Immune Activation Profiles

Polymeric nanoparticles (NPs) have emerged as promising tools for immunomodulation in various disease contexts. The interactions between the NP surface and plasma-resident biomolecules result in the formation of a biomolecular corona (BC), which varies patient-to-patient and as a function of disease state, resulting in the concept of the personalized NP-BC. This study investigates how the progression of systemic inflammatory disease influences the NP-BC composition and its corresponding effects on innate immune cell interactions and activation profiles. Employing a murine model of systemic inflammation, the dynamic changes in plasma biomolecule composition and their corresponding NP-BC fingerprints were elucidated. An integrated multi-omics approach was developed to reveal disease state-specific alterations in innate immune cell phenotypes and modulated signaling pathways to identify and confirm key circulating biomolecules that contribute to the dynamic immunostimulatory capabilities of BCs. In this dataset, high throughput targeted and quantitative metabolomic analysis was carried out using the Biocrates MxP Quant 500 kit (Biocrates, Life Science AG, Innsbruck, Austria). The MxP Quant 500 kit was prepared following the instructions of the manufacturer.