Expression test and purification of SETDB1 catalytic domain constructs' for structural studies by X-ray crystallography.
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<strong>Experiment: </strong>Expression test and purification of SETDB1 catalytic domain constructs’ for structural studies by X-ray crystallography. <strong>Aim:</strong> In the present section of this study, we focused on the development of efficient bacterial expression systems to produce large amounts of soluble SETDB1 catalytic domain for structural studies. This report involves a summary of expression test results of different fragments of SETDB1 and purification of various fusion proteins.
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2019-10-02
