Peptide library purity by mass spectrometry (Coassolo et al., 2023)

The library was produced as crude peptides at a quantity of 1-4mg with a C-terminal amidation as the only modification for comparative studies between peptides. Identity and purity of the peptides used in vivo were verified by LC-MS using an Agilent Q-TOF LC–MS instrument. The raw mass spectrometry (MS1 spectra) data are deposited as Agilent .d file for the six synthesized peptides used in the in vivo screen (BRINP2_peptide 5.d, EDIL3_peptide 5.d, FGF3_peptide 4.d, FGF5_peptide 5.d, FSTL4_peptide 3.d and SCG1_peptide 9.d).