Data from: Interacting proteome of PtDAL10 and PtDAL1 in Pinus tabuliformis

The optimal timing of the transition from vegetative growth to
reproductive growth is critical for plant reproductive success, and the
underlying regulatory mechanisms have been well studied in angiosperm
model species, but relatively little in gymnosperms. Recently, DAL1, a
MADS domain transcription factor (TF) gene that shows a conserved
age-related expression profile in conifers, was found to serve as an age
timer. However, how the DAL1 mediates the onset of reproductive growth
remains poorly understood. Here, we have shown that the PtDAL1 directly
regulates the PtDAL10 transcription by binding to its promoter region,
resulting in a ternary MADS-domain TF complex with PtDAL10 and PtMADS11,
two potential candidate regulators of the vegetative to reproductive
transition in Chinese pine (Pinus tabuliformis). The PtDAL10 was
progressively induced in new shoots with age and highly accumulated in
male and female cones. Overexpression of PtDAL10 rescued the flowering of
ft-10 and soc1-1-2 mutants in Arabidopsis. We provide insight into the
molecular components associated with the PtDAL1, which integrates the
vegetative to reproductive phase transition into age-mediated progressive
development of the whole plant in conifers.