Trapping efficiency of peptides on particle-packed and micro-pillar trap columns for proteomics analyses

In current work, we compared the separation of HeLa samples using two different trap columns for sample trapping, desalting, and preconcentration. The two columns have different types of the C18 stationary phase: a) a conventional packed PepMap C18 trap-column (Thermo Fischer Scientific), and b) the superficially porous silicon pillars μPAC C18 trap-column (PharmaFluidics)