Yield and purity estimates from fed-batch fermentor production of the MSP-1 hybrid.
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Total protein yields were calculated by BCA assay on samples from each purification step. The specific MSP-1 hybrid content in each sample was determined by sandwich ELISA, using monoclonal 12.2 as a capture antibody and anti-MSP-1 hybrid polyclonal rabbit IgG as a detection reagent. MSP-1 hybrid content in samples from each purification step was calculated by interpolation from a standard curve, using serial dilutions of purified MSP-1 hybrid as capture antigen. Purity and yield were then calculated from these two measurements.
创建时间：
2015-12-02
