Genome-wide maps of Meis1 and Hoxb13 chromatin binding in murine heart

A major factor in the progression to heart failure in humans is the inability of the adult heart to repair itself after injury. As a result, strategies to induce heart regeneration have received significant interest. We reported that transcription factors Meis1 and Hoxb13 contributes to postnatal cardiomyocyte cell cycle exit, and inhibiting these genes expression promote adult cardiomyocyte regeneration. In order to indentify the downstream targets of these transcription factors we generated ChIP-seq of mouse heart for Meis1 and Hoxb13. The results demonstrate that Meis1 and Hoxb13 act cooperatively to regulate cardiomyocyte maturation and proliferation and provide insights into the link between hyperplastic and hypertrophic growth of cardiomyocytes. Overall design: Examination of Meis1 and Hoxb13 binding site in the murine heart