MSC-NK co-culture, D12, MSC2
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http://flowrepository.org/id/FR-FCM-ZYA7
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资源简介:
NK cells from an healthy donor (D12) were co-cultured for 4 days with human lung tumor-derived MSC and non-tumor MSC, and activated toward K562/primary tumor cells for 4 hours. NK degranulation (CD107a) and IFNgamma production were assessed by flow cytometry. Gating strategy for NK cells: CD45+CD3-CD56+CD16+/-. Inhibitors were used to block MSC activity.
创建时间:
2017-08-01



