Differential Expression of miRNAs in control and NR_034085-silenced OSCCs under cisplatin treatment

chemoresistance frequently leads to therapeutic failure in oral squamous cell carcinoma (OSCC). Recently, studies have reported that lncRNAs play active roles in regulation biological properties of cancer and may regulate the expression of miRNA. We also found that miRNAs could regulate cisplatin sensitivity in TSCC previously. In this study, to explore the potential targets of lncRNAs in response to cisplatin, we performed microarray to detect miRNAs both in control and NR_034085–silenced CAL-27 and SCC-9 cells under cisplatin treatment. We investigated the differential expression profiles of miRNA in tongue squamous carcinoma cells between 6 groups: the CAL27 control group, the CAL27 stably transduced with shRNA1 targeted NR_034085 (CAL27 shRNA1 group), the CAL27 stably transduced with shRNA3 targeted NR_034085 (CAL27 shRNA3 group), the SCC9 control group and the SCC9 stably transduced with shRNA1 targeted NR_034085 (SCC9 shRNA1 group), the SCC9 stably transduced with shRNA3 targeted NR_034085 (SCC9 shRNA3 group).Sample labeling and array hybridization were performed by Agilent_Human_miRNA_8x60k_70156_v21 (Agilent Technologies) according to the Agilent miRNA Microarray protocol. After quantile normalization of the raw data, the miRNAs detected were chosen for further data analysis. Differentially expressed miRNAs with statistical significance between the two groups were identified through P value/FDR filtering.