Single cell RNA sequencing of total peritoneal cells from Beclinf/f-Lyz2cre mice

In Beclinf/f-Lyz2cre mice, we observed proinflammatory activation of tissue-resident peritoneal macrophages. These mice were resistent to lethal bacterial infection with Listeria. It is important to understand how the peritoneal immunity is affected by Beclin deletion in myeloid cells. To characterize the immune cells in the peritoneum, we performed single cell RNA sequencing of total peritoneal cells from Beclinf/f-Lyz2cre and littermate control Beclinf/f mice. The single cell RNA-seq analyses revealed profound changes in both the myeloid compartment and bystander cells. Overall design: Total peritoneal cells from Becn1f/f-LysMcre and Becn1f/f mice, n=4 each, age 8 wks